Posted: July 15th, 2022

Describe key functions of the basement membrane

Describe key functions of the basement membrane

Question Assignment 1

a) In the chemical fixation step of an H&E protocol describe why perfusion of a tissue is most popular over immersion.

b) You obtain a freshly ready slide of a liver tissue that a lab colleague ready with an H&E protocol. Upon putting it below the microscope you discover that the integrity of the cells are completely conserved however that there’s near no distinction all through the slide. Your colleague is 100% sure that he used the appropriate dyes and also you belief his certainty on that matter. Contemplating the above info, what did your lab mate do flawed?

c) Present a ONE sentence description of immunohistochemistry and supply a brief (2 sentences max) clarification of the scientific ideas on which it’s based mostly.

Question Assignment 2

a. Describe the 5 key functions of the basement membrane and why ailments affecting its integrity are of an enormous concern.

b. Describe the two constitutive parts of a proteoglycan. Describe how a proteoglycan differs from a glycoprotein. Present a brief description of the proteoglycan particular to the basement membrane and its operate it participates.

c. Through which layer of the epithelium are stem cells situated? Present the two organic rules (Time & Amount) that govern the proliferation of these stem cells.

d. Present a proof as to why cells are handled with taxol (a microtubule stabilizing drug) don’t full mitosis and state the stage of mitosis by which these cells will stay.

Question Assignment three

Medical histology can be utilized for numerous functions, e.g. observing the state of a diseased organ, assessing the construction of an organ considered dysfunctional, trying to find the manifestation of parasite contamination , and many others.. Go to a scientific search engine (ex: PubMed) and discover a analysis article printed in the final three years that makes use of histology.

– Clarify why the investigators used a histological method on this publication (how was it helpful, how will it reply the goal/Question Assignment of the investigation, and why not use one other method)

– Describe the protocol used for this histological preparation (is it commonplace H&E, produce other stains been used, was immunohistochesmitry used…)

– Add a figures of this publication which exhibits the histological outcomes and talk about how this determine suits in the publication (in phrases of the outcomes it presents, and the way it addresses the Question Assignment the investigators have been out to reply)

References: All web sites, papers, and textbooks have to be referenced in the textual content and in a bibliography at the finish. *Observe- Wikipedia and your professor’s lecture notes are NOT acceptable references!

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